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East Africa Region

IPM CRSP International Stakeholder Meeting:
Assessing Plant Disease Diagnostic Capacity and Needs in East Africa

Workshop: Plant Disease Diagnostic Technology Training
IPM CRSP International Plant Diagnostic Network (IPDN)
March 5-9, 2007; Nairobi, Kenya

AGENDA

DAY 1 Monday, March 5: ARRIVAL AND ORIENTATION
2.30 p.m. Arrival and registration of participants at NARL Conference Hall (KARI-NARL, Waiyaki Way, Nairobi)
3.30 p.m. Orientation and briefing of participants by hosting scientists
4.30 p.m. End
   
DAY 2 Tuesday, March 6: STAKEHOLDERS' MEETING
8.15 Arrival and registration (continuation) at the venue (NARL Conference Hall)

  Session 1: Opening and Introductory presentations
Chair: Dr. Joseph Muriithi, Deputy Director-R&T, KARI
Rapporteurs: Dr. E.M. Ateka & Dr. Ruth Amata

8.45 Official Opening
- Welcome address: Dr. P. T. Gicheru, Centre Director - KARI-NARL
- Introductions and Overview: Mrs. Mary Wabule, AD-HIC, KARI
- Stakeholders' meeting objectives: Dr. Sally Miller, The Ohio State University
- USAID official address: Dr. Peter Ewell / Mr. A. Fleming
- Ministry of Agric (Kenya) address: Dr. Wilson Songa, Agriculture Secretary

- Opening Speech: Dr. Ephraim Mukisira, Director KARI
10.00 IPM activities in East Africa: Dr. Mark Erbaugh, The Ohio State University, USA
10.15 Tea/coffee break

Session 2: Diagnostic Capacity, Needs and Networking
Chair: Dr. Josephine Songa, Crop Protection Coordinator, KARI
Rapporteurs: Dr. E.M. Ateka & Dr. Ruth Amata

10.45 The IPM CRSP Insect-Transmitted Viruses Project: James Legg, IITA
11.10 Sanitary/phytosanitary practices and compliance in East Africa: Dr. C.J. Kedera, MD-KEPHIS
11.40 Plant disease diagnostic capacity and needs in the floriculture industry: Jane Ngigi, Kenya Flower Council
11.55 Plant disease diagnostic capacity and needs in vegetable and fruit production: Dr. S. Mbithi, FPEAK
12:10 IPDN activities in the West Africa region- the West Africa Diagnostic Network: Dr. Fen Beed, IITA
12.25 Databases and communication: the planned IPDN system: Dr. Sally Miller, The Ohio State University, USA
12.55 Plant disease diagnostic capacity assessment: Dr. Sally Miller, The Ohio State University, USA (Distribution of survey documents)
13.05 Lunch break

Session 3: Modeling Plant Disease Diagnostic Networks
Chair: Dr. Josephine Songa, Crop Protection Coordinator, KARI
Rapporteurs: Dr. E.M. Ateka & Dr. Ruth Amata

14.00  Institutional plant disease diagnostic capacity and needs: 15 minute presentations by representatives of East African institutions
  • Kenya - Dr. Z.M. Kinyua and Prof. G. Siboe
  • Uganda - MAAIF/NARO/Makerere University
  • Tanzania - Ministry of Agriculture/Sokoine University
  • KEPHIS - Dr. Esther W. Kimani
15.15  Positioning of diagnostic support and regional centres of excellence:
Dr Julian Smith, Central Science Laboratories, UK
15.30 Discussions: Modeling of a functional Regional Plant Disease Diagnostic Network.
16.30 Tea/coffee break
17.00 End

WORKSHOP: PLANT DISEASE DIAGNOSTIC TECHNOLOGY TRAINING

DAY 3 Wednesday, 7 March
 
  Session 4: Basic Principles of Plant Disease Diagnosis (1)
Chair:
Dr. Geoffrey Tusiime, Makerere University
Rapporteurs:
Mr. Martin Lagat & Irene Njagi
8.30 Use of serology in plant disease diagnostics: Dr. Sally Miller
9.15 Use of PCR in plant disease diagnostics: Dr. Doug Maxwell
10.00 Coffee/Tea Break
10.30 Diagnosis of bacteria and phytoplasmas by classical and modern techniques: Dr. Sally Miller and Dr. Julian Smith
11.15 Diagnosing virus diseases - approaches suited to available technology: Dr. E.M. Ateka
12.00 An overview of state-of-the-art Information and Communication Technologies for Biodiversity and Plant Diagnostics: Dr. Fen Beed
13.00 Lunch break
 
  Session 5: Bacteriology & phytoplasmas practicals (14.00 - 17.00 hrs)

Session organisers: Drs. Sally Miller, Z.M. Kinyua, & Geoffrey Tusiime
Sample cases: Banana xanthomonas wilt, potato bacterial wilt and napier grass phytoplasma
Aspects to be covered: symptoms, isolation/culturing (semi-selective media), PCR and ELISA.

Session 6: Virus disease diagnostic practicals (14.00 - 17.00 hrs)
Session organisers:
Drs. Douglas Maxwell, James Legg, IITA & E.M. Ateka

Sample cases: Geminiviruses and Aphid-transmitted viruses
Aspects to be covered: symptoms, extraction, PCR, serology, etc.
DAY 4 Thursday, 8 March
8.15 Continuation of sessions 5 and 6 (Swapping of groups)
11.00 Coffee/Tea Break
 
  Session 7: Basic Principles of Plant Disease Diagnosis (2)
Chair:
Dr. Simon T. Gichuki, KARI
Rapporteurs: Dr. Margaret Mulaa & Mr. Moses Nyongesa
11.30 Nematode diagnostics - Dr. J.W. Kimenju
12.15 Fungal & Oomycete disease diagnostics - Prof. George Siboe & Prof. Eunice Mutitu
13.00 Lunch break
 
  Session 8: Nematode diagnostic practicals (14.00 - 17.00 hrs)
Session organisers:
Dr. J.W. Kimenju and Miriam Otipa

Sample cases: Root knot and lesion nematodes
Aspects to be covered: symptoms, extraction, histology, microscopy, etc.

  Session 9: Fungal/oomycete diagnostic practicals (14.00 - 17.00 hrs)
Session organisers:
Prof. G. Siboe, Dr. Ruth Amata and Prof E. Mutitu

Sample cases: Vascular wilts, root rots, and blights
Aspects to be covered: symptoms, isolation, microscopy, DNA analyses, etc.
DAY 5 Friday, 9 March
8.15 Continuation of sessions 8 and 9 (Swapping of groups)
11.00 Coffee/Tea Break
 
  Session 10: Utilizing Internet-based Resources in Diagnostics

Chair: Dr. Anne Wangai
Rapporteurs: J.K. Sitieney & Mrs. Miriam Otipa
11:30 Interpreting DNA sequence information: Dr. Doug Maxwell, Univ. of Wisconsin-Madison
12.30 Discussions
13.00 Lunch break
 
  Session 11: Wrap-up and presentation of certificates
Facilitators: Dr. Anne Wangai and Dr. Sally Miller
Rapporteurs: J.K. Sitieney & Mrs. Miriam Otipa
14.00 Wrap-up and discussions on the way forward
16.15 Presentation of certificates
16.30 Closing Remarks: Dr. Wilson Songa, Agriculture Secretary, Kenya.
Dr. Sally Miller
Department of Plant Pathology
The Ohio State University – OARDC
1680 Madison Avenue Wooster, OH 44691
Ph: 330-263-3678 Fax: 330-263-3678
E-mail: miller.769@osu.edu