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East Africa Region
IPM CRSP International
Stakeholder Meeting:
Assessing Plant Disease Diagnostic Capacity and Needs in
East Africa
Workshop: Plant Disease Diagnostic Technology Training
IPM CRSP International Plant Diagnostic Network (IPDN)
March 5-9, 2007; Nairobi, Kenya
AGENDA
| DAY 1 |
Monday, March 5: ARRIVAL AND ORIENTATION |
| 2.30 p.m. |
Arrival and registration of participants at NARL
Conference Hall (KARI-NARL, Waiyaki Way, Nairobi) |
| 3.30 p.m. |
Orientation and briefing of participants by hosting
scientists |
| 4.30 p.m. |
End |
| |
|
| DAY 2 |
Tuesday, March 6: STAKEHOLDERS' MEETING |
| 8.15 |
Arrival and registration (continuation) at the venue
(NARL Conference Hall)
| |
Session 1: Opening and Introductory presentations
Chair: Dr. Joseph Muriithi, Deputy Director-R&T, KARI
Rapporteurs: Dr. E.M. Ateka & Dr. Ruth Amata |
|
| 8.45 |
Official Opening
- Welcome address: Dr. P. T. Gicheru, Centre Director - KARI-NARL
- Introductions and Overview: Mrs. Mary Wabule, AD-HIC, KARI
- Stakeholders' meeting objectives: Dr. Sally Miller, The Ohio State University
- USAID official address: Dr. Peter Ewell / Mr. A. Fleming
- Ministry of Agric (Kenya) address: Dr. Wilson Songa, Agriculture Secretary
- Opening Speech: Dr. Ephraim Mukisira, Director KARI |
| 10.00 |
IPM activities in East Africa: Dr. Mark Erbaugh, The Ohio State
University, USA |
| 10.15 |
Tea/coffee break
|
Session 2: Diagnostic Capacity, Needs and Networking
Chair: Dr. Josephine Songa, Crop Protection Coordinator, KARI
Rapporteurs: Dr. E.M. Ateka & Dr. Ruth Amata |
|
| 10.45 |
The IPM CRSP Insect-Transmitted Viruses Project: James Legg, IITA |
| 11.10 |
Sanitary/phytosanitary practices and compliance in East Africa:
Dr. C.J. Kedera, MD-KEPHIS |
| 11.40 |
Plant disease diagnostic capacity and needs in the floriculture industry:
Jane Ngigi, Kenya Flower Council |
| 11.55 |
Plant disease diagnostic capacity and needs in vegetable and fruit
production: Dr. S. Mbithi, FPEAK |
| 12:10 |
IPDN activities in the West Africa region- the West Africa Diagnostic
Network: Dr. Fen Beed, IITA |
| 12.25 |
Databases and communication: the planned IPDN system: Dr. Sally Miller,
The Ohio State University, USA |
| 12.55 |
Plant disease diagnostic capacity assessment: Dr. Sally Miller, The Ohio
State University, USA (Distribution of survey documents) |
| 13.05 |
Lunch break
|
Session 3: Modeling Plant Disease Diagnostic Networks
Chair: Dr. Josephine Songa, Crop Protection Coordinator, KARI
Rapporteurs: Dr. E.M. Ateka & Dr. Ruth Amata |
|
| 14.00 |
Institutional plant disease diagnostic capacity and needs: 15 minute
presentations by representatives of East African institutions
- Kenya - Dr. Z.M. Kinyua and Prof. G. Siboe
- Uganda - MAAIF/NARO/Makerere University
- Tanzania - Ministry of Agriculture/Sokoine University
- KEPHIS - Dr. Esther W. Kimani
|
| 15.15 |
Positioning of diagnostic support and regional centres of excellence:
Dr Julian Smith, Central Science Laboratories, UK |
| 15.30 |
Discussions: Modeling of a functional Regional Plant Disease Diagnostic
Network. |
| 16.30 |
Tea/coffee break |
| 17.00 |
End |
WORKSHOP: PLANT DISEASE DIAGNOSTIC TECHNOLOGY TRAINING
| DAY 3 |
Wednesday, 7 March
| |
Session 4: Basic Principles of Plant Disease Diagnosis (1)
Chair: Dr. Geoffrey Tusiime, Makerere University
Rapporteurs:
Mr. Martin Lagat & Irene
Njagi |
|
|
8.30 |
Use of serology in plant disease diagnostics: Dr. Sally Miller
|
|
9.15 |
Use of PCR in plant disease diagnostics: Dr. Doug Maxwell |
|
10.00 |
Coffee/Tea Break |
|
10.30 |
Diagnosis of bacteria and phytoplasmas by classical and modern techniques:
Dr. Sally Miller and Dr. Julian Smith |
|
11.15 |
Diagnosing virus diseases - approaches suited to available technology: Dr. E.M. Ateka |
|
12.00 |
An overview of state-of-the-art Information and Communication Technologies
for Biodiversity and Plant Diagnostics: Dr. Fen Beed |
|
13.00 |
Lunch break
| |
Session 5: Bacteriology & phytoplasmas practicals (14.00
- 17.00 hrs)
Session organisers: Drs. Sally Miller, Z.M. Kinyua, & Geoffrey Tusiime
Sample cases: Banana xanthomonas wilt, potato bacterial wilt and napier grass
phytoplasma
Aspects to be covered: symptoms, isolation/culturing (semi-selective media), PCR
and ELISA. |
|
Session 6: Virus disease diagnostic practicals (14.00
- 17.00 hrs)
Session organisers: Drs. Douglas Maxwell, James Legg, IITA & E.M. Ateka
Sample cases: Geminiviruses and Aphid-transmitted viruses
Aspects to be covered: symptoms, extraction, PCR, serology, etc. |
|
| DAY 4 |
Thursday, 8 March |
|
8.15 |
Continuation of sessions 5 and 6 (Swapping of groups) |
|
11.00 |
Coffee/Tea Break
| |
Session 7: Basic Principles of Plant Disease Diagnosis (2)
Chair: Dr. Simon T. Gichuki, KARI
Rapporteurs: Dr. Margaret Mulaa & Mr. Moses Nyongesa |
|
|
11.30 |
Nematode diagnostics - Dr. J.W. Kimenju |
|
12.15 |
Fungal & Oomycete disease diagnostics - Prof. George Siboe & Prof. Eunice
Mutitu |
|
13.00 |
Lunch break
| |
Session 8: Nematode diagnostic practicals (14.00
- 17.00 hrs)
Session organisers: Dr. J.W. Kimenju and Miriam Otipa
Sample cases: Root knot and lesion nematodes
Aspects to be covered: symptoms, extraction, histology, microscopy, etc. |
| |
Session 9: Fungal/oomycete diagnostic practicals (14.00
- 17.00 hrs)
Session organisers: Prof. G. Siboe, Dr. Ruth Amata and Prof E. Mutitu
Sample cases: Vascular wilts, root rots, and blights
Aspects to be covered: symptoms, isolation, microscopy, DNA analyses, etc. |
|
| DAY 5 |
Friday, 9 March |
|
8.15 |
Continuation of sessions 8 and 9 (Swapping of groups) |
|
11.00 |
Coffee/Tea Break
| |
Session 10: Utilizing Internet-based Resources in Diagnostics
Chair: Dr. Anne Wangai
Rapporteurs: J.K. Sitieney & Mrs. Miriam Otipa |
|
|
11:30 |
Interpreting DNA sequence information: Dr. Doug Maxwell,
Univ. of Wisconsin-Madison |
|
12.30 |
Discussions
|
|
13.00 |
Lunch break
| |
Session 11: Wrap-up and presentation of certificates
Facilitators: Dr. Anne Wangai and Dr. Sally Miller
Rapporteurs: J.K. Sitieney & Mrs. Miriam Otipa |
|
|
14.00 |
Wrap-up and discussions on the way forward
|
|
16.15 |
Presentation of certificates
|
|
16.30 |
Closing Remarks: Dr. Wilson Songa, Agriculture Secretary, Kenya.
|
|
Dr. Sally Miller
Department of Plant Pathology
The Ohio State University – OARDC
1680 Madison Avenue Wooster, OH 44691
Ph: 330-263-3678 Fax: 330-263-3678
E-mail: miller.769@osu.edu


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